B20 bayanus grown at 20 C B25 bayanus grown at 25 C B30 bayanus grown at 30 C Bayanus Heat Shock (17-30) 5m Bayanus Heat Shock (17-30) 15m Bayanus Heat Shock (17-30) 30m Bayanus Heat Shock (17-30) 60m Bayanus Heat Shock (25-37) 0min Bayanus Heat Shock (25-37) 5min Bayanus Heat Shock (25-37) 15min Bayanus Heat Shock (25-37) 30min Bayanus Heat Shock (25-37) 60min Bayanus Heat Shock (25-42) 0m Bayanus Heat Shock (25-42) 5m Bayanus Heat Shock (25-42) 15m Bayanus Heat Shock (25-42) 30m Heat Shock 0' (25-37) Heat Shock 5' (25-37) heat shock 15' (25-37) Heat Shock 30' (25-37) Heat shock 45' (25-37) sorbitol 0 mins sorbitol 5 mins sorbitol 10 mins sorbitol 15 mins sorbitol 20 mins sorbitol 30 mins Ethanol Exposure (0 min.) Ethanol Exposure (5 min.) Ethanol Exposure (10 min.) Ethanol Exposure (20 min.) Ethanol Exposure (30 min.) Ethanol Exposure (60 min.) Ammonium Exposure (0 min.) Ammonium Exposure (5 min.)(2) Ammonium Exposure (10 min.) Ammonium Exposure (20 min.) Ammonium Exposure (30 min.) Ammonium Exposure (60 min.) Sulfite Exposure (0 min.) Sulfite Exposure (5 min.) Sulfite Exposure (10 min.) Sulfite Exposure (20 min.) Sulfite Exposure (30 min.) Sulfite Exposure (60 min.) Cadmium Exposed (0 min.) Cadmium Exposed (5 min.) Cadmium Exposed (20 min.) Cadmium Exposed (30 min.) Copper Exposed (0 min.) Copper Exposed (5 min.) Copper Exposed (10 min.) Copper Exposed (20 min.) Copper Exposed (30 min.) Copper Exposed (60 min.) Lead Exposure (0 min.) Lead Exposure (5 min.) Lead Exposure (10 min.) Lead Exposure (20 min.) Lead Exposure (30 min.) Lead Exposure (60 min.) Nickel Exposure (0 min.) Nickel Exposure (5 min.) Nickel Exposure (20 min.) Nickel Exposure (30 min.) Nickel Exposure (60 min.) Zinc Exposure (0 min.) Zinc Exposure (5 min.) Zinc Exposure (10 min.) Zinc Exposure (20 min.) Zinc Exposure (30 min.) Zinc Exposure (60 min.) Hydrogen Peroxide Exposure (0 min.) Hydrogen Peroxide Exposure (5 min.) Hydrogen Peroxide Exposure (10 min.) Hydrogen Peroxide Exposure (20 min.) Hydrogen Peroxide Exposure (30 min.) Hydrogen Peroxide Exposure (60 min.) Peroxide Challenge 0 oxidative stress time point Time Point 10 (peroxide) Peroxide Challenge 15 Peroxide Challenge 20 Peroxide Challenge 30 0minH2O2 5minH2O2 10minH2O2 Peroxide Treatment 15 min Peroxide Treatment 20 min Peroxide Treatment 30 min Bleach Exposure (.25%, 0 min.) Bleach Exposure (.25%, 5 min.) Bleach Exposure (.25%, 10 min.) Bleach Exposure (.25%, 20 min.) Bleach Exposure (2, .25%, 30 min.) Bleach Exposure (2, .25%, 60 min.) mms 0 min mms 5 min mms 10 min mms 15 min mms 20 min mms 30 min zeocin 0 mins zeocin 5 mins zeocin 10 mins zeocin 15 mins zeocin 20 mins zeocin 30 mins 0 minute hydroxyurea Hydroxyurea (5 minutes) Hydroxyurea (10 minute) 15 minute hydroxyurea Hydroxyurea (20 minute) Hydroxyurea (30 minute) tunicamycin treatment (t=0) Tunicamycin treatment (t = 5 min) Tunicamycin Treatment TimePoints t=10 Tunicamycin Treatment time t=15 Timepoint 20min after treatment with Tunicamycin (2mg/mL in DMSD) Timepoint 30min after treatment with Tunicamycin (2mg/mL in DMSD) MG-132 0 mins MG-132 10 mins MG-132 15 mins MG-132 20 mins MG-132 30 mins LovastatinT0 Lovastatin Time Series - 5 Minutes LovastatinT20 Lovastatin Time Series - 30 Minutes rapamycin 0 min rapamycin 5 mins rapamycin 10 mins rapamycin 15 mins rapamycin 20 mins rapamycin 30 mins 2-deoxyglucose 0 min 2-deoxyglucose 5 min 2-deoxyglucose 10 min 2-deoxyglucose 15 min. Again 2-deoxyglucose 20 min 2-deoxy-glucose, 30 min. Acy3 exponential ACY3 T0 Acy3 2 hrs Acy3 4hrs. Acy3 6 Days ACY4 T0 ACY4 2hrs new ACY4 4hrs new Acy4 1 day new Acy7 exponential Acy7 0+2 hrs. Acy7 1day Acy7 day 6 Diauxic Shift 1 Diauxic Shift 2 Diauxic Shift 3 Diauxic Shift 4 Diauxic Shift 5 Diauxic Shift 6 galactose 0 min galactose 15 mins galactose 60 mins galactose 90 mins galactose 210 mins sucrose 0 min glycerol 15 min glycerol 60 min glycerol 90 min glycerol 210 min sucrose 15 mins sucrose 60 mins sucrose 210 mins Lys Limit - 13.5 hr. Lys Limit - 15 hr. Lys Limit - 16.5 hr. Lys Limit - 18 hr. Tryp Limit - 11 hr. Tryp Limit - 13.5 hr. Tryp Limit - 16.5 hr. Tryp Limit - 19 hr. Ura Limit - 11 hr. Ura Limit - 13.5 hr. Ura Limit - 16.5 hr. BB11 Sulfur-lim-30c Vs RNA 5/13 BB11 Glucose-lim-25c BB11 Glucose-lim-30c BB11 Phosphate-25c BB11 Phosphate(bad)-lim-30c BB11 low potassium(DH)-lim-25c BB11 high potassium(DH)-lim-25c BB11 (diploid) bayanus a mating type bayanus alpha mating type bayanus Alpha- factor II- 0 min Alpha- factor II- 5 min Alpha- factor II- 15 min Alpha- factor II- 30 min Alpha- factor II- 45 min Alpha- factor II- 60 min Alpha- factor II- 90 min Alpha- factor II- 120 min 0 time point cell cycle of S.B n 10 time point cell cycle of S.B n 20 time point cell cycle of S.B 40 time point cell cycle of S.B fixed 50 time point cell cycle of S.B n 60 time point cell cycle of S.B n 70 time point cell cycle of S.B n 80 time point cell cycle of S.B 90 time point cell cycle of S.B 100 time point cell cycle of S.B 110 time point cell cycle of S.B 120 time point cell cycle of S.B fixed 130 time point cell cycle of S.B f 140 time point cell cycle of S.B fixed 150 time point cell cycle of S.B fixed 160 time point cell cycle of S.B fixed 170 time point cell cycle of S.B 180 time point cell cycle of S.B 190 time point cell cycle of S.B 200 time point cell cycle of S.B 210 time point cell cycle of S.B 220 time point cell cycle of S.B 230 time point cell cycle of S.B 240 time point cell cycle of S.B 250 time point cell cycle of S.B time point cell cycle of S.B -1 260 min time point cell cycle of S.B -2 270 min time point cell cycle of S.B -3 280 min time point cell cycle of S.B -4 290 min time point cell cycle of S.B -5 300 min 24hrs time point sporulation of S.b. BB10 26.5hrs time point sporulation of S.b. BB10 30.5hrs time point sporulation of S.b. BB10 33hrs time point sporulation of S.b. BB10 35hrs time point sporulation of S.b. BB10 37hrs time point sporulation of S.b. BB10 39hrs time point sporulation of S.b. BB10 46.5hrs time point sporulation of S.b. BB10 48hrs time point sporulation of S.b. BB10 16hrs time point sporulation of S.b 24hrs time point sporulation of S.b 40hrs time point sporulation of S.b 48hrs time point sporulation of S.b BB11 64hrs time point sporulation of S.b BB11 16hrs time point sporulation of S.b NRRL-Y11845 24hrs time point sporulation of S.b NRRL-Y11845 40hrs time point sporulation of S.b NRRL-Y11845 64hrs time point sporulation of S.b NRRL-Y11845 BB11 vs. CY1 log growth CY1 vs. CY1 log growth DBY10075 vs. CY1 log growth NRRL-Y11845 vs. CY1 log growth NRRL-Y11845 vs. Mixed Reference YZB5-102 vs. Mixed Reference 8D A4 vs. Mixed Reference 6B A3 vs. Mixed Reference 8D A5 vs. Mixed Reference 8D A3 vs. Mixed Reference 6B A1 vs. Mixed Reference 6B A5 vs. Mixed Reference 6B A4 vs. Mixed Reference 8D A2 vs. Mixed Reference 6B A2 vs. Mixed Reference 8D A10 vs. Mixed Reference 8D A1 vs. Mixed Reference 6B A7 vs. Mixed Reference 8D A9 vs. Mixed Reference 8D A8 vs. Mixed Reference 6B A8 vs. Mixed Reference 6B A6 vs. Mixed Reference 6B A10 vs. Mixed Reference 8D A6 vs. Mixed Reference 6B A9 vs. Mixed Reference 8D A7 vs. Mixed Reference S. bayanus Tn insert 14 vs. Mixed Reference S. bayanus Tn insert 19 vs. Mixed Reference S. bayanus Tn insert 21 vs. Mixed Reference S. bayanus Tn Insert 7 vs. Mixed Reference S. bayanus Tn Insert 8 vs. Mixed Reference S. bayanus Tn Insert 2 vs. Mixed Reference S. bayanus Tn insert 32 vs. Mixed Reference S. bayanus Tn insert 22 vs. Mixed Reference S. bayanus Tn insert 24 vs. Mixed Reference S. bayanus Tn insert 25 vs. Mixed Reference S. bayanus Tn insert 33 vs. Mixed Reference S. bayanus Tn insert 27 vs. Mixed Reference S. bayanus Tn insert 28 vs. Mixed Reference S. bayanus Tn insert 36 vs. Mixed Reference S. bayanus Tn insert 26 vs. Mixed Reference S. bayanus Tn insert 15 vs. Mixed Reference S. bayanus Tn insert 16 vs. Mixed Reference S. bayanus Tn insert 20 vs. Mixed Reference S. bayanus Tn insert 18 vs. Mixed Reference S. bayanus Tn Insert 6 vs. Mixed Reference S. bayanus Tn insert 12 vs. Mixed Reference S. bayanus Tn Insert 3 vs. Mixed Reference S. bayanus Tn insert 11 vs. Mixed Reference S. bayanus Tn Insert 1 vs. Mixed Reference S. bayanus Tn Insert 9 vs. Mixed Reference S. bayanus Tn insert 10 vs. Mixed Reference S. bayanus Tn insert 34 vs. Mixed Reference ACY331 0 min ACY331 30 min ACY331 60 min ACY678 0 min ACY678 30 min ACY678 60 min galactose Acy7 0 min galactose Acy7 15 min galactose Acy7 30 min galactose Acy7 45 min galactose Acy7 65 min galactose Acy7 90 min galactose Acy7 110 min galactose Acy7 145 min galactose Acy7 175 min galactose Acy703 0 min galactose Acy703 15 min galactose Acy703 30 min galactose Acy703 45 min galactose Acy703 65 min galactose Acy703 90 min galactose Acy703 110 min galactose Acy703 145 min galactose Acy703 175 min galactose Acy732 0 min galactose Acy732 15 min galactose Acy732 30 min galactose Acy732 45 min galactose Acy732 65 min galactose Acy732 90 min galactose Acy732 110 min galactose Acy732 145 min galactose Acy732 175 min 555.11 Segregant 1 (Alpha) 555.11 Segregant 2 (Alpha) 555.11 Segregant 3 (A) 555.11 Segregant 4 (A) 670.20 Segregant (A1) 670.20 Segregant (B1) 670.20 Segregant (C1) 670.20 Segregant (D1)