Heat Shock 10 minutes (expt 1) Heat Shock 15 minutes (expt 1) Heat Shock 20 minutes (expt 1) Heat Shock 30 minutes (expt 1) Heat Shock 40 minutes (expt 1) Heat Shock 60 minutes (expt 1) Heat Shock 80 minutes (expt 1) Heat Shock 000 minutes (expt 2) Heat Shock 000 minutes (expt 2) Heat Shock 000 minutes (expt 2) Heat Shock 005 minutes (expt 2) Heat Shock 015 minutes (expt 2) Heat Shock 030 minutes (expt 2) Heat Shock 060 minutes (expt 2) 37C to 25C shock - 15 min 37C to 25C shock - 30 min 37C to 25C shock - 45 min 37C to 25C shock - 60 min 37C to 25C shock - 90 min heat shock 17 to 37, 20 minutes heat shock 21 to 37, 20 minutes heat shock 25 to 37, 20 minutes heat shock 29 to 37, 20 minutes heat shock 33 to 37, 20 minutes 29C to 33C - 5 minutes 29C to 33C - 15 minutes 29C to 33C - 30 minutes 33C vs. 30C - 90 minutes 29C +1M sorbitol to 33C + 1M sorbitol - 5 minutes 29C +1M sorbitol to 33C + 1M sorbitol - 15 minutes 29C +1M sorbitol to 33C + 1M sorbitol - 30 minutes 29C +1M sorbitol to 33C + *NO sorbitol - 5 minutes 29C +1M sorbitol to 33C + *NO sorbitol - 15 minutes 29C +1M sorbitol to 33C + *NO sorbitol - 30 minutes constant 0.32 mM H2O2 (10 min) constant 0.32 mM H2O2 (20 min) constant 0.32 mM H2O2 (30 min) constant 0.32 mM H2O2 (40 min) constant 0.32 mM H2O2 (50 min) constant 0.32 mM H2O2 (60 min) constant 0.32 mM H2O2 (80 min) constant 0.32 mM H2O2 (100 min) constant 0.32 mM H2O2 (120 min) constant 0.32 mM H2O2 (160 min) 1 mM Menadione (10 min) 1 mM Menadione (20 min) 1 mM Menadione (30 min) 1mM Menadione (40 min) 1 mM Menadione (50 min) 1 mM Menadione (80 min) 1 mM Menadione (105 min) 1 mM Menadione (120 min) 1 mM Menadione (160 min) 2.5mM DTT 005 min (expt 1) 2.5mM DTT 015 min (expt 1) 2.5mM DTT 030 min (expt 1) 2.5mM DTT 045 min (expt 1) 2.5mM DTT 060 min (expt 1) 2.5mM DTT 090 min (expt 1) 2.5mM DTT 120 min (expt 1) 2.5mM DTT 180 min (expt 1) 2.5 mM DTT 000 min (expt 2) 2.5 mM DTT 015 min (expt 2) 2.5 mM DTT 030 min (expt 2) 2.5 mM DTT 060 min (expt 2) 2.5 mM DTT 120 min (expt 2) 2.5 mM DTT 240 min (expt 2) 2.5 mM DTT 480 min (expt 2) 1.5 mM diamide (5 min) 1.5 mM diamide (10 min) 1.5 mM diamide (20 min) 1.5 mM diamide (30 min) 1.5 mM diamide (40 min) 1.5 mM diamide (50 min) 1.5 mM diamide (60 min) 1.5 mM diamide (90 min) 1M sorbitol - 5 min 1M sorbitol - 15 min 1M sorbitol - 30 min 1M sorbitol - 45 min 1M sorbitol - 60 min 1M sorbitol - 90 min 1M sorbitol - 120 min Hypo-osmotic shock - 5 min Hypo-osmotic shock - 15 min Hypo-osmotic shock - 30 min Hypo-osmotic shock - 45 min Hypo-osmotic shock - 60 min steady-state 1M sorbitol aa starv 0.5 h aa starv 1 h aa starv 2 h aa starv 4 h aa starv 6 h Nitrogen Depletion 30 min. Nitrogen Depletion 1 h Nitrogen Depletion 2 h Nitrogen Depletion 4 h Nitrogen Depletion 8 h Nitrogen Depletion 12 h Nitrogen Depletion 1 d Nitrogen Depletion 2 d Nitrogen Depletion 3 d Nitrogen Depletion 5 d Diauxic Shift Timecourse diauxic shift timecourse diauxic shift timecourse diauxic shift timecourse diauxic shift timecourse diauxic shift timecourse diauxic shift timecourse diauxic shift timecourse YPD stationary phase 2 h (expt 2) YPD stationary phase 4 h (expt 2) YPD stationary phase 6 h (expt 2) YPD stationary phase 8 h (expt 2) YPD stationary phase 10 h (expt 2) YPD stationary phase 12 h (expt 2) YPD stationary phase 1 d (expt 2) YPD stationary phase 2 d (expt 2) YPD stationary phase 3 d (expt 2) YPD stationary phase 5 d (expt 2) YPD stationary phase 2 h (expt 1) YPD stationary phase 4 h (expt 1) YPD stationary phase 8 h (expt 1) YPD stationary phase 12 h (expt 1) YPD stationary phase 1 d (expt 1) YPD stationary phase 2 d (expt 1) YPD stationary phase 3 d (expt 1) YPD stationary phase 5 d (expt 1) YPD stationary phase 7 d (expt 1) YPD stationary phase 13 d (expt 1) YPD stationary phase 22 d (expt 1) YPD stationary phase 28 d (expt 1) wildtype 37degree heat - 20 min msn2-4- 37degree heat - 20 min msn2/4 + 37degrees (20 min) yap1- 37degree heat - 20 min yap1 + 37degree heat wildtype + 0.3 mM H2O2 (20 min) msn2msn4 + 0.32 mM H2O2 msn2/4 + 0.32 mM H2O2 (20 min) yap1- + 0.3 mM H2O2 (20 min) yap1 + 0.32 mM H2O2 (20 min) Msn2 overexpression Msn4 overexpression YAP1 overexpression YP ethanol (expt 1) YP galactose (expt 1) YP glucose (expt 1) YP mannose (expt 1) YP raffinose (expt 1) YP sucrose (expt 1) YP ethanol (expt 2) YP fructose (expt 2) YP galactose (expt 2) YP glucose (expt 2) YP mannose (expt 2) YP raffinose (expt 2) YP sucrose (expt 2) steady state 17C (expt 1) steady state 21C (expt 1) steady state 25C (expt 1) steady state 29C (expt 1) steady state 37C (expt 1) steady state 15 C (expt 2) steady state 17 C (expt 2) steady state 21 C (expt 2) steady state 25 C (expt 2) steady state 29 C (expt 2) steady state 33 C (expt 2) steady state 36 C (expt 2) steady state 36 C (expt 2)